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Establishment of an IL‐2‐dependent cell line derived from ‘nasal‐type’ NK/T‐cell lymphoma of CD2 + , sCD3 − , CD3ɛ + , CD56 + phenotype and associated with the Epstein‐Barr virus
Author(s) -
Kagami Yoshitoyo,
Nakamura Shigeo,
Suzuki Ritsurou,
Iida Shinsuke,
Yatabe Yasushi,
Okada Yasutaka,
Kobayashi Tomoko,
Tsurumi Tatsuya,
Seto Masao,
Ogura Michinori,
Taguchi Osamu,
Morishima Yasuo
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.01029.x
Subject(s) - biology , lymphoma , microbiology and biotechnology , epstein–barr virus , granzyme b , t cell lymphoma , cell culture , cd3 , t cell , natural killer cell , antigen , cd8 , virus , cytotoxic t cell , virology , immunology , immune system , in vitro , genetics
A novel interleukin‐2 (IL‐2)‐dependent cell line, HANK1, was established from a patient with CD56 + NK/T‐cell lymphoma arising in the retroperitoneum. Morphologically, HANK1 is a pleomorphic large cell line with irregular nuclei, which contains azurophilic granules in the cytoplasm. Immunophenotypic analysis showed that HANK1 expressed CD2, CD3ɛ, CD56, TIA‐1, granzyme B, and HLA‐DR, but no other T‐lineage markers. These features were the same as seen in the original tumour, and are highly characteristic of nasal and ‘nasal‐type’ NK/T‐cell lymphoma as described in the proposed W.H.O. classification. Genotypically, this cell line also demonstrated the germline configuration of the T‐cell receptor β, γ and the immunoglobulin heavy chain genes and clonal integration of the Epstein‐Barr virus (EBV) together with antigen expression with a type II latency pattern (LMP‐1 + and EBNA2 − ). Furthermore, Southern blot analysis using the EBV termini as probes confirmed its derivation from the original lymphoma, and revealed that it contained multiple copies of the EBV genome. Dose‐dependent growth on IL‐2 was observed in an in vitro study with a doubling time of 3 d at maximal stimulation. These data indicate that HANK1 seemed to preserve the biological characteristics of the original tumour and therefore may serve as a good model for the further analysis of unusual ‘nasal‐type’ NK/T‐cell lymphoma.