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Bioactive substances in buffy‐coat‐derived platelet pools stored in platelet‐additive solutions
Author(s) -
Edvardsen Lisbeth,
Taaning Ellen,
Mynster Tommie,
Hvolris Jesper,
Drachman Ole,
Nielsen Hans JØrgen
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.00990.x
Subject(s) - buffy coat , platelet , extracellular , histamine , chemistry , myeloperoxidase , andrology , platelet activation , immunology , biochemistry , pharmacology , biology , medicine , inflammation
We studied extracellular accumulation in buffy‐coat‐derived platelet pools suspended in platelet additive solutions (PAS). 20 platelet pools were prepared from buffy coats of 60 donors and stored in either PAS I ( n = 10) or PAS II ( n = 10). Samples were drawn from the donors to determine the normal range of histamine, plasminogen activator inhibitor 1 (PAI‐1), myeloperoxidase (MPO) and interleukin (IL)‐6. After preparation samples were collected on days 0, 5 and 7 from the 20 platelet pools respectively, to determine extracellular accumulation of the same substances. Extracellular histamine, PAI‐1 and MPO were detected in significant levels ( P < 0.0001) immediately after preparation of the platelet pools. Only the extracellular level of PAI‐1 increased significantly during storage from day 0 to day 7 ( P < 0.01). After preparation and storage to day 7 IL‐6 was found in only three of the 20 platelet pools. Even though platelets were prepared by the buffy‐coat method, to produce a low leucocyte count and thereby low cytokine (IL‐6) content, there was still significant contamination by other bioactive substances.