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Low CD4 counts rather than superantigenic‐like effects account for differences in expressed T‐cell receptor (TCR) repertoires between HIV‐1 seropositive long‐term non‐progressors and individuals with progressive disease
Author(s) -
Westby Michael,
Vaughan Andrew N,
Balotta Claudia,
Galli Massimo,
Clerici Mario,
Dalgleish Angus G
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.00912.x
Subject(s) - t cell receptor , biology , superantigen , seroconversion , immunology , virology , repertoire , immune system , viral disease , virus , t cell , physics , acoustics
Analysis of HIV‐infected individuals who have stable CD4 counts many years after seroconversion may provide an insight as to how the host's immune system can successfully control HIV infection. In this study we analysed the T‐cell receptor (TCR) Vβ repertoire in 13 HIV + individuals, seven of whom were classed as long‐term non‐progressors (LTNP), using a technique which couples anchor PCR (AnPCR) amplification of β‐chain cDNA to differential probe hybridization with non‐radioactively labelled Vβ family specific oligonucleotide probes. There were no significant differences in the expressed TCR repertoires between the LTNP group and the other HIV‐infected individuals. However, there was a statistically significant inverse correlation between CD4 count and the number of Vβ family‐specific perturbations in the recent seroconverters (SC) and those with progressive infection (PI), consistent with other shared features of clinical disease progression (Th1/Th2 switch and high frequency of viral isolation). We conclude that long‐term clinical non‐progression in HIV‐1 infection is not associated with the loss or expansion of a particular Vβ family; instead, low CD4 count in the PI and SC individuals was correlated with increased number of Vβ family‐specific perturbations relative to the LTNP group and that it is hence unlikely that HIV encodes a superantigen.