Premium
Establishment and characterization of a mantle cell lymphoma cell line
Author(s) -
Jeon HO Jong,
Kim Chul Woo,
Yoshino Tadashi,
Akagi Tadaatsu
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.00911.x
Subject(s) - mantle cell lymphoma , cd5 , cyclin d1 , cd19 , microbiology and biotechnology , epstein–barr virus , cd23 , blastoid , cell culture , cd43 , immunophenotyping , biology , lymphoma , virology , cd20 , cell , flow cytometry , virus , antibody , immunology , cell cycle , genetics , immunoglobulin e
A mantle cell lymphoma (MCL) cell line (JeKo‐1) was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukaemic conversion. JeKo‐1 cells were Epstein‐Barr virus negative and showed a B‐cell phenotype with IgM + , IgD + , CD3 − , CD5 + , CD10 − , CD19 + , CD20 + and CD23 − ; they overexpressed cyclin Dl, Bcl‐2, c‐Myc and Rb proteins. Bcl ‐1/J H gene rearrangement was confirmed by polymerase chain reaction, although karyotypic analysis showed 40/41 chromosomes devoid of apparent t(11;14)(q13;q32) translocation. JeKo‐1 cells were highly tumourigenic in SCID mice.