Premium
Bb1‐3, a transgenic hybrid cell line with erythroid and megakaryocytic differentiation potential that expresses high levels of human γ‐globin and human β‐globin
Author(s) -
Thomson Andrew M.,
Roberts Nigel A.,
Wood William G.
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.00864.x
Subject(s) - biology , globin , cellular differentiation , microbiology and biotechnology , cell culture , megakaryocyte , transgene , erythropoiesis , gata1 , genetically modified mouse , lineage markers , haematopoiesis , progenitor cell , gene , stem cell , genetics , anemia , medicine
We have characterized a murine hybrid cell line, Bb1‐3, generated by the fusion of mouse primary erythroblasts with MEL cells. It proliferated in serum‐free medium and displayed a low level of spontaneous erythroid and megakaryocyte differentiation. Terminal erythroid differentiation could be induced with HMBA and DMSO and was enhanced by serum. Treatment with phorbol esters resulted in a high proportion of megakaryocytes and the expression of megakaryocytic specific lineage markers. Bb1‐3 cells contain a human β‐globin transgene that was expressed at levels of 20–50% of the endogenous mouse globin genes. Initially, expression was largely limited to the β‐globin gene but after adaptation to serum free growth, equal expression of both the human γ‐ and human β‐globin genes was observed. This cell line provides further evidence that the differentiation potential of mouse erythroleukaemia cells is not restricted to the erythroid lineage and should be useful to study the mechanisms underlying both developmental globin gene regulation and the terminal differentiation of bipotential erythroid/megakaryocytic progenitor cells.