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A novel surface marker (B203.13) of human haemopoietic progenitors, preferentially expressed along the B and myeloid lineages
Author(s) -
Zamai Loris,
V Marco,
Bennett Ian M.,
Croce Carlo M.,
Perussia Bice
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.00856.x
Subject(s) - myeloid , progenitor cell , biology , cd34 , monoclonal antibody , bone marrow , stem cell , immunology , microbiology and biotechnology , haematopoiesis , cd33 , cord blood , antibody
We used a monoclonal antibody (mAb) (B203.13, IgM) generated from a mouse immunized with the human B/myeloid bi‐phenotypic B1b cell line, to analyse haemopoietic cells. The antigen recognized by this mAb is expressed on most adult and umbilical cord blood CD21 + B cells, at minimal density on mature monocytes, and is undetectable on granulocytes, T, natural killer (NK) cells, and erythrocytes. Within umbilical cord blood and adult bone marrow haemopoietic progenitor cells, the B203.13 mAb recognized a surface marker, present on progenitor cells of several haemopoietic lineages, that was transiently expressed on early erythroid and T/NK progenitors, and was preferentially maintained on cells of the B and myeloid lineages. Within the CD34 + cells, B203.13 was expressed on early committed myeloid (CD33 + ) and erythroid (CD71 dim ) progenitor cells, as confirmed in colony formation assays. The mAb also reacted with cells of B and myeloid chronic leukaemias and cell lines. These data define B203.13 mAb as a novel reagent useful for the characterization of haemopoietic progenitors and leukaemias.