Premium
Two M‐components in a single cell lineage in a patient with a dual isotype secretory B‐cell tumour
Author(s) -
Sakashita Chizuko,
Saito Takako,
Kurosu Tetsuya,
Yoshinaga Haruhiko,
Kumagai Takasi,
Yamamoto Koh,
Miki Tohru,
Koyama Takatoshi,
Miura Osamu,
Nemoto Tetsuo,
Asakawa Hideo,
Hirosawa Shinsaku
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.00832.x
Subject(s) - immunoglobulin light chain , isotype , biology , antibody , bone marrow , microbiology and biotechnology , gene , immunofluorescence , homology (biology) , population , immunoglobulin class switching , b cell , immunology , genetics , medicine , monoclonal antibody , environmental health
We describe a case of Waldenström's macroglobulinaemia with two M‐components (IgM and IgG) with the same λ light chain. Southern blot analysis of bone marrow cells showed rearrangements of immunoglobulin heavy and λ light chain genes. Sequencing of the complementarity determining region 3 of the two γ and μ transcripts showed 100% homology. Immunofluorescence study showed that most cells stained for both IgG and IgM. These findings indicated that a single population of cells was expressing two isotypic variants of IgG and IgM, as the genes responsible for production of both components had the same origin.