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Collection of Ph‐negative progenitor cells with granulocyte‐colony stimulating factor in patients with chronic myeloid leukaemia who respond to recombinant alpha‐interferon
Author(s) -
Reiffers,
Simon Taylor,
Gluckman,
Gorin,
Simon J. Mahon,
MiclÉa,
DestrÉe,
) Gautier
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.00821.x
Subject(s) - leukapheresis , medicine , granulocyte colony stimulating factor , cd34 , progenitor cell , peripheral blood mononuclear cell , granulocyte , myeloid , alpha interferon , interferon alfa , immunology , bone marrow , gastroenterology , interferon , chemotherapy , stem cell , biology , in vitro , biochemistry , genetics
This study aimed to demonstrate that sufficient Ph‐negative blood progenitors could be collected following administration of glycosylated rhG‐CSF (lenograstim) to patients with Philadelphia chromosome (Ph)‐positive chronic myeloid leukaemia (CML) who responded to recombinant alpha‐interferon (α‐IFN) (Ph‐positive marrow metaphases < 35%). 23 patients received lenograstim (150 μg/m 2 ) once daily for a median of 9 d. Peak circulating numbers of white blood cells (36.4 × 10 9 /l), CD34 + cells (24/μl) and colony‐forming unit‐granulocyte‐macrophage (CFU‐GM; 1346.5/ml) occurred at a median of day 8, day 8 and day 7, respectively. Two to six (median three) leukaphereses (LK) were performed from days 5 to 12. The median number of mononuclear cells (MNC), CD34 + cells and CFU‐GM collected per patient was 7.35 × 10 8 /kg, 2.72 × 10 6 /kg and 10.23 × 10 4 /kg, respectively. 22/23 patients had LK which contained either 10 4 CFU‐GM/kg and/or 10 6 CD34 + cells/kg; 47 LK (from 20/22 patients) were evaluable for cytogenetics. The median percentage of Ph‐positive cells was 0, and 43/47 LK (91%) contained < 35% Ph‐positive cells; 25 (53%) were entirely negative. Sixteen of 20 evaluable patients had one or more LK with < 35% Ph‐positive cells, and 12 had at least one 100% Ph‐negative LK. Mobilization and collection of Ph‐negative cells were not influenced by the dose or duration of α‐IFN administration before (or during) lenograstim administration or by the quality of cytogenetic response (complete v major) during lenograstim administration. No significant side‐effects were observed. Thus, lenograstim administration can result in satisfactory Ph‐negative blood progenitor cell collection. Autologous transplantation of such cells may be used when indicated.