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Associations between TGF‐β1 receptors in human bone marrow stromal cells
Author(s) -
Robledo Mar M.,
Ursa Ma Angeles ,
SánchezMadrid Francisco,
TeixidÓ JoaquÍn
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.00820.x
Subject(s) - stromal cell , endoglin , cytokine , bone marrow , receptor , transforming growth factor , microbiology and biotechnology , biology , chemistry , cancer research , immunology , stem cell , biochemistry , cd34
Bone marrow stromal cells are required for sustained haemopoiesis. Transforming growth factor‐β (TGF‐β) is a multifunctional cytokine present in the bone marrow microenvironment which regulates the expression of several cytokines, cytokine receptors and cell adhesion elements. The TGF‐β receptors type I and II, and endoglin, mediate TGF‐β1 binding to the membrane of human bone marrow stromal cells. [ 125 I]TGF‐β1‐affinity labelling experiments showed that three different anti‐endoglin monoclonal antibodies co‐immunoprecipitated a 68 kD TGF‐β1‐labelled polypeptide together with TGF‐β1/endoglin complexes. Here, we have shown that the 68 kD receptor corresponds to the type I receptor, indicating that endoglin and the type I receptor associate on the membrane of these cells upon ligand binding. The expression of endoglin by stromal cells was found to be up‐regulated by TGF‐β1, but not by IL‐1β. The association of endoglin with signalling components of the TGF‐β receptor system on the membrane of bone marrow stromal cells might modulate TGF‐β1 access to the signalling pathways, and therefore it could regulate TGF‐β1‐mediated stromal cellular responses.