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Similar characteristics of the CDR3 of V H 1‐69/DP‐10 rearrangements in normal human peripheral blood and chronic lymphocytic leukaemia B cells
Author(s) -
Brezinschek HansPeter,
Brezinschek Ruth I,
DÖrner Thomas,
Lipsky Peter E
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.00787.x
Subject(s) - complementarity determining region , biology , chronic lymphocytic leukemia , gene , antigen , gene rearrangement , microbiology and biotechnology , b cell , peripheral blood , breakpoint cluster region , immunoglobulin light chain , antibody , immunology , genetics , leukemia
The variable heavy chain (V H ) gene segment V H 1‐69/DP‐10 has been shown to be over‐represented in B‐cell chronic lymphocytic leukaemia (CLL). Because of certain similar characteristics of their complementarity determining region 3 (CDR3), including preferential utilization of J H 6 elements and an extended length, it has been suggested that antigenic stimulation might be involved in leukaemogenesis. Utilizing single‐cell PCR to amplify and sequence genomic DNA from individual normal human peripheral blood B cells, we have obtained 7/421 productively and 1/69 nonproductively rearranged V H genes that used V H 1‐69/DP‐10. All productive rearrangements were unmutated, used J H 6 and had an average CDR3 length similar to that previously found in V H 1‐69/DP‐10‐expressing CLL cells. These results suggest that CLL may arise from B cells commonly found in the peripheral B‐cell repertoire and do not represent expansion of a unique subset of specific antigen‐reactive B cells.