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DNA extraction from archival Giemsa‐stained bone‐marrow slides: comparison of six rapid methods
Author(s) -
Vince Adriana,
Poljak Mario,
Seme Katja
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.00702.x
Subject(s) - isoamyl alcohol , giemsa stain , dna extraction , chromatography , extraction (chemistry) , proteinase k , polymerase chain reaction , distilled water , boiling , dna , chemistry , bone marrow , microbiology and biotechnology , biology , pathology , medicine , alcohol , biochemistry , organic chemistry , gene
The ability of six rapid DNA extraction procedures to provide DNA for the polymerase chain reaction from archival Giemsa‐stained bone marrow slides was tested on 120 samples. Boiling in distilled water, freeze–thaw method, boiling in 10% Chelex‐100 resin solution, proteinase K/Tween 20/NP‐40 method coupled with simplified phenol/chloroform/isoamyl alcohol protocol or salting‐out procedure using saturated NaCl and modification of commercial QIAamp procedure (Qiagen, Chatsworth, Calif.) gave DNA extraction efficiencies of 50%, 70%, 85%, 95%, 100% and 100%, respectively. Our results demonstrate that rough DNA extraction methods have decreased efficiencies compared to complete DNA extraction protocols and that the latter are required to ensure highly reproducible results from archival Giemsa‐stained bone marrow slides.