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Progenitor cell subsets and engraftment kinetics in children undergoing autologous peripheral blood stem cell transplantation
Author(s) -
GonzalezRequejo Angel,
Madero Luis,
DÍaz Miguel A.,
Villa Marta,
GarcÍAEscribano Carmen,
Balas Antonio,
Lillo Rosa,
GarcÍASanchez Félix,
Benito Ana,
Vicario Jose L.
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.00662.x
Subject(s) - leukapheresis , cd38 , cd34 , stem cell , transplantation , immunology , progenitor cell , flow cytometry , apheresis , andrology , biology , medicine , platelet , microbiology and biotechnology
The main objective of the present study was to determine the role of CD34 + cell subsets in the haemopoietic recovery of children undergoing peripheral blood stem cell transplantation. For this purpose, 38 leukaphereses from 33 children with malignancies mobilized with G‐CSF were analysed. Using dual‐colour flow cytometry, different subpopulations of CD34 + cells were quantified and the number of each reinfused subsets correlated with haemopoietic resurgence. Multivariate analysis showed that the number of CD34 + CD38 − cells and CD34 + CD38 + cells correlated better with time to neutrophil and platelet recovery, respectively, than the total number of CD34 + cells. Threshold values for rapid haemopoietic recovery, determined by the receiver operating characteristic analysis, were found to be 0.5 × 10 6 CD34 + CD38 − cells for neutrophil engraftment, and 2.0 × 10 6 CD34 + CD38 + cells for platelet recovery. It is suggested that the analysis of CD34 + cell subsets could increase understanding of the repopulation capacity of a given leukapheresis product in peripheral blood stem cell transplantation procedures in children. In particular, this procedure could be extremely useful when low numbers of CD34 + cells are collected.