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The Platelet Function Analyzer (PFA‐100 ® ): a novel in‐vitro system for evaluation of primary haemostasis in children
Author(s) -
Manuel Carção,
Victor S. Blanchette,
J.A. Dean,
Lijing He,
Marlena Kern,
Ann Marie Stain,
Catherine Sparling,
Derek Stephens,
Gerard Ryan,
John Freedman,
Margaret L. Rand
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.00656.x
Subject(s) - platelet , medicine , von willebrand disease , blood sampling , haemophilia , hemostasis , in vitro , von willebrand factor , surgery , immunology , chemistry , biochemistry
The PFA‐100 ® system provides an in‐vitro method of assessing primary platelet‐related haemostasis by measuring the time (the closure time, or CT) taken for a platelet plug to occlude a microscopic aperture cut into a membrane coated with collagen and either epinephrine or ADP. We used the system to establish normal ranges for CTs in healthy children, adults and neonates. Mean CTs of healthy children were independent of the needle gauge used (21G or 23G) for blood sampling; they were very similar to the mean CTs of healthy adults, but longer than mean CTs of healthy neonates. Although children with haemophilia had normal CTs, the PFA‐100 system was found to be potentially useful in screening for von Willebrand disease in children.