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Complementary and antagonistic effects of IL‐3 in the early development of human megakaryocytes in culture
Author(s) -
Dolzhanskiy Alexandr,
Hirst John,
Basch Ross S.,
Karpatkin Simon
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.00579.x
Subject(s) - thrombopoietin , endoreduplication , megakaryocyte , biology , microbiology and biotechnology , haematopoiesis , platelet , cell culture , chemistry , immunology , andrology , cell , stem cell , biochemistry , cell cycle , genetics , medicine
The effect of IL‐3 on the early steps in the growth and development of megakaryocytes (MK) in culture has been studied. Although thrombopoietin (TPO) by itself could support the development of mature CD41 + MK from pre‐MK, the number of cells produced was greatly augmented by the addition of IL‐3 and SCF. IL‐3 was also able to support the growth of MK colonies in semi‐solid media (CFU‐MK). The CD41 + cells that developed in suspension cultures containing IL‐3 differed phenotypically from those that developed without this agent. Cells grown in the presence of IL‐3 lost CD34 expression more rapidly, expressed lower levels of the platelet glycoproteins gpIIb‐IIIa and Ib and achieved lower degrees of polyploidy than in the absence of IL‐3. The inhibitory effects of IL‐3 were not a consequence of the dilution of the mature cells by increased numbers of immature cells since it was observed under conditions in which IL‐3 did not stimulate MK growth. The results obtained in these cultures suggest that IL‐3 plays an important role in early MK development, but inhibits further maturation after endoreduplication begins. Thus, prolonged contact with IL‐3 results in the appearance of cells that do not mature normally.