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Megakaryocyte progenitors derived from bone marrow or G‐CSF‐mobilized peripheral blood CD34 + cells show a distinct phenotype and responsiveness to interleukin‐3 (IL‐3) and PEG‐recombinant human megakaryocyte growth and development factor (PEG‐rHuMGDF)
Author(s) -
Catani Lucia,
Gugliotta Luigi,
Campanini Elena,
Mangianti Serena,
Gibellini Davide,
Baravelli Stefano,
Vianelli Nicola,
Lemoli Roberto Massimo,
Tura Sante
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.00511.x
Subject(s) - megakaryocyte , bone marrow , cd34 , progenitor cell , peripheral blood mononuclear cell , interleukin 3 , haematopoiesis , stem cell , granulocyte colony stimulating factor , biology , immunology , microbiology and biotechnology , medicine , t cell , interleukin 21 , immune system , in vitro , chemotherapy , biochemistry
In the present study we investigated the proliferative response of megakaryocyte progenitor cells (CFU‐MK) derived from peripheral blood stem cell (PBSC) collections of patients with haematological malignancies and normal donors. Highly purified CD34 + cells and mononuclear cell fractions were assayed in the presence of recombinant interleukin‐3 (IL‐3) and pegylated‐recombinant human megakaryocyte growth and development factor (PEG‐rHuMGDF), alone or in combination, and megakaryocyte colony formation was evaluated in the plasma clot. In comparison, steady‐state bone marrow samples from normal donors were highly enriched in CD34 + cells and tested with the cytokines studied. Our results showed that IL‐3 was able to stimulate CFU‐MK colony formation from bone marrow and peripheral blood CD34 + cells. Similarly, PEG‐rHuMGDF stimulated, in a dose–response manner, CD34 + cells from the bone marrow. However, normal mobilized peripheral blood CD34 + cells were not induced to generate CFU‐MK colonies by PEG‐rHuMGDF. The same lack of response was observed when patients peripheral blood CD34 + cells primed with chemotherapy plus G‐CSF or with G‐CSF alone were assessed. In contrast, PEG‐rHuMGDF stimulated CFU‐MK growth when mononuclear cells, either from the bone marrow or from mobilized peripheral blood, were grown in plasma clot. Moreover, we analysed by flow cytometry the expression of Mpl receptor on the cell membrane of normal mobilized peripheral blood and normal steady‐state bone marrow CD34 + cells. Our results showed a reduced expression of Mpl receptor on mobilized peripheral blood progenitor cells in comparison with bone marrow cells.