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Flow cytometric method for the routine follow‐up of red cell populations after bone marrow transplantation
Author(s) -
Hendriks E. C. M.,
De Man A. J. M.,
Van Berkel Y. C. M.,
Stienstra S.,
De Witte T.
Publication year - 1997
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1997.d01-2138.x
Subject(s) - bone marrow transplantation , medicine , bone marrow , flow cytometry , hematopoietic cell , transplantation , pathology , immunology , biology , stem cell , haematopoiesis , genetics
A simple and sensitive flow cytometric method is presented for the quantitation of erythrocyte subpopulations. Cells were indirectly labelled using human antisera (mostly crude) and fluorescein isothiocyanate (FITC)‐conjugated anti‐human IgG F ab . The method was evaluated by analysing artificial mixtures of blood group antigens A, B, D, E, c, K and Fy a . Intra‐assay coefficients of variation were found to be 7.2% for 1% D‐positive mixtures and 2.2% for 10% D‐positive mixtures; the inter‐assay coefficients of variation were 9.5% and 6.0% respectively. The sensitivity of the method was found to be 0.31%. The method has shown to be suitable for the routine follow‐up of patients after allogeneic bone marrow transplantation (BMT).