Premium
Action of human interleukin‐4 and stem cell factor on erythroid and mixed colony formation by peripheral blood‐derived CD34 + c‐kit high or CD34 + c‐kit low cells
Author(s) -
SONODA YOSHIAKI,
KIMURA TAKAFUMI,
OHMIZONO YOSHIKAZU,
SAKABE HIDEAKI,
TANIMUKAI SHIGEATSU,
YOKOTA SHOUHEI,
CLARK STEVEN C.,
ABE TATSUO
Publication year - 1997
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1997.d01-2091.x
Subject(s) - stem cell factor , cd34 , progenitor cell , erythropoietin , population , stem cell , interleukin 3 , microbiology and biotechnology , chemistry , biology , immunology , endocrinology , t cell , medicine , interleukin 21 , immune system , environmental health
We studied the interaction of interleukin (IL)‐4 and other burst‐promoting activity (BPA) factors, such as IL‐3, granulocyte/macrophage colony‐stimulating factor (GM‐CSF), IL‐9 and stem cell factor (SCF), on erythroid burst‐forming unit (BFU‐E) and erythrocyte‐containing mixed (CFU‐Mix) colony formation in serum‐free culture. IL‐4 alone did not support mixed colony formation in the presence of erythropoietin (Epo). However, IL‐4 showed weak but significant BPA when peripheral blood (PB)‐derived CD34 + c‐kit low cells were used as the target population. The BPA of IL‐4 was much weaker than that of IL‐3, which exerted the most potent activity, as previously reported. When CD34 + c‐kit high cells were used as the target, four factors known to have BPA, IL‐3, GM‐CSF, IL‐9 and SCF, could express BPA. In contrast, IL‐4 alone failed to support erythroid burst formation. Interestingly, IL‐4 showed a remarkable enhancing effect with SCF in promoting the development of erythroid burst and erythrocyte‐containing mixed colonies from CD34 + c‐kit low and CD34 + c‐kit high cells. Delayed addition of SCF + Epo or IL‐4+Epo to the cultures initiated with either IL‐4 or SCF alone clearly demonstrated that SCF was a survival factor for both BFU‐E and CFU‐Mix progenitors. In contrast, the survival effect of IL‐4 was much weaker than that of SCF, and appeared to be more important for progenitors derived from CD34 + c‐kit low cells than for those derived from CD34 + c‐kit high cells. It was recently reported that CD34 + c‐kit low cells represent a more primitive population than CD34 + c‐kit high cells. Taken together, these results suggest that IL‐4 helps to recruit primitive progenitor cells in the presence of SCF.