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Administration of G‐CSF to healthy subjects: the effects on eosinophil counts and mobilization of eosinophil granule proteins
Author(s) -
KARAWAJCZYK M.,
HO¨GLUND M.,
ERICSSON J.,
VENGE P.
Publication year - 1997
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1997.d01-2045.x
Subject(s) - eosinophil , eosinophil cationic protein , eosinophil peroxidase , eosinophil granule proteins , medicine , immunology , major basic protein , white blood cell , endocrinology , eosinophilia , asthma
Any influence of G‐CSF on eosinophils is mostly negative, although reports which have studied this relationship are few with varied results. The aim of this study was to investigate the influence of G‐CSF administration to healthy subjects on eosinophils in peripheral blood. Blood eosinophil counts, serum levels of eosinophil cationic protein (ECP), eosinophil peroxidase (EPO) and eosinophil protein X (EPX), as well as cell morphology were studied. 14 healthy volunteers received 7.5 μg ( n = 8) or 10 μg/kg body weight ( n = 6) G‐CSF daily for six consecutive days. ECP and EPX were assessed by specific RIAs and EPO by a specific FEIA. Cell morphology was examined by electron microscopy. During G‐CSF administration, eosinophil counts increased from 0.22 ±0.04 ×10 9 /l to 0.61 ± 0.098 × 10 9 /l ( P = 0.001), serum ECP from 12.39 ± 2.45 μg/l to 61.82 ± 7.38 μg/l ( P = 0.0014), serum EPX from 28.05 ±4.54 μg/l to 87.96 ±9.84 μg/l ( P =0.002) and serum EPO from 8.89 ±2.2 μg/l to 19.98 ± 5.1 μg/l ( P = 0.003). All variables returned gradually to initial values after discontinuation of G‐CSF. Distinct changes in the morphology of secondary granules were observed 24 h after G‐CSF administration. The granules became irregular and their matrix less electron dense. We conclude that administration of G‐CSF to healthy humans increases the number of circulating eosinophils and affects the mobilization of eosinophil granule proteins.