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Efficient RT‐PCR on platelet mRNA after long‐term storage
Author(s) -
PEYRUCHAUD Olivier,
NURDEN Alan,
Bourre François
Publication year - 1997
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1997.d01-2004.x
Subject(s) - messenger rna , platelet , microbiology and biotechnology , protein subunit , chemistry , coding region , receptor , thrombin , biology , gene , immunology , biochemistry
We have developed a procedure permitting RT‐PCR from mRNA even after a long‐term storage (1 year) of platelet samples in ethanol (EtOH‐platelets) at −80°C. To validate our method, we have analysed the human platelet alloantigen system (HPA‐1) which is coded by β 3 mRNA. We have also demonstrated the efficiency of amplification of part of the coding region for (i) α IIb subunit mRNA, (ii) α v subunit mRNA, and (iii) the seven transmembrane domain thrombin receptor mRNA.