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Concordant expression of class II and class III CD34 epitopes on haemopoietic cells in leukapheresis and cord blood samples with CD45/CD34 dual staining
Author(s) -
Rudin Claudius E.,
Garland John M.,
Joyner Miles V.
Publication year - 1997
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1997.602716.x
Subject(s) - cord blood , leukapheresis , cd34 , epitope , microbiology and biotechnology , progenitor cell , biology , flow cytometry , clone (java method) , immunology , stem cell , antibody , genetics , gene
We used flow cytometry with CD45/CD34 dual antibody labelling to investigate the relative expression of class II and class III CD34 epitopes on haemopoietic progenitor cells (HPC) in 22 leukapheresis and cord blood samples. There was a close correlation between class II (QBEnd‐10) and class III (clone 581) CD34 + cells ( R 2 = 0.975, ratio of class III to class II CD34 + count 1.24 ±0.04). The linear relationship between class II and III CD34 epitopes on HPC suggests that the choice of antibody class is not a source of random variation in the quality assurance of CD34 + cell enumeration.