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Quantitative characterization and potential function of membrane Fas/APO‐1 (CD95) receptors on leukaemic cells from chronic B and T lymphoid leukaemias
Author(s) -
Kamihira Shimeru,
Yamada Yasuaki,
Hirakata Yoichi,
Tsuruda Kazuto,
Sugahara Kazuyuki,
Tomonaga Masao,
Maeda Takahiro,
Tsukasaki Kunihiro,
Atogami Sunao,
Kobayashi Nobuyuki
Publication year - 1997
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1997.4963301.x
Subject(s) - fas receptor , fas ligand , apoptosis , chronic lymphocytic leukemia , biology , antibody , b cell , monoclonal antibody , immune system , microbiology and biotechnology , programmed cell death , receptor , immunology , leukemia , biochemistry
The expression and function of the Fas‐receptor (Fas‐R) were examined in chronic lymphocytic leukaemia (CLL), hairy cell leukaemia‐variant (HCL‐v) and adult T‐cell leukaemia (ATL). The expression of Fas‐R in freshly isolated leukaemic cells was qualitatively and quantitatively different between each disease; faint in B‐CLL, moderate in HCL‐v and strong in ATL. Both full‐length and alternatively spliced truncated forms of Fas mRNA were detected even in CLL B cells with faint to negative Fas‐R, and Fas mRNA was also shown to be capable of increasing in vitro expression, i.e. the message was functional. In contrast, Fas‐R expression on ATL cells was heterogenous and usually intense with a mean density approximately 3‐fold higher than that of normal T cells. Fas‐R was confirmed to have the potential function for anti‐Fas monoclonal antibody‐mediated cell death in vitro in Fas‐R + ATL cells. The expression level of Fas‐R on the cells was higher in chronic than acute ATL (10360 v 6260 antibody‐binding capacity per cell, mFas ABC ; P < 0.05) and was inversely correlated with serum LDH activity, suggesting that the strong Fas‐R accounts for the slow progression of chronic ATL and the negative Fas‐R protects from Fas‐mediated cell death. These results show that Fas‐R expression on leukaemic cells is valuable in their characterization and perhaps their function, and may contribute to the progression and immune evasion of malignant clones.