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Tropism of human herpesvirus 8 for peripheral blood lymphocytes in patients with Castleman's disease
Author(s) -
Kikuta Hideaki,
Itakura Osamu,
Taneichi Koji,
Kohno Michifumi
Publication year - 1997
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1997.4653269.x
Subject(s) - castleman disease , medicine , lymphoproliferative disorders , peripheral blood mononuclear cell , virology , tropism , pathology , lymphocyte , lymphoid hyperplasia , polymerase chain reaction , immunology , virus , disease , lymphoma , biology , biochemistry , gene , in vitro
Multicentric Castleman's disease (MCD), also called multicentric angiofollicular lymphoid hyperplasia, is a systemic lymphoproliferative disorder causing fever, lymphadenopathy and splenomegaly. Recently, Kaposi's sarcoma‐associated herpesvirus/human herpesvirus 8 (KSHV/HHV‐8) DNA sequences have been detected in cases of MCD. We examined HHV‐8 DNA sequences in the peripheral blood mononuclear cells (PBMCs) of two HIV‐negative patients with MCD and in PBMCs and the lymph node of a HIV‐negative patient with localized Castleman's disease (LCD) by the polymerase chain reaction. The novel sequences were detected in all DNA samples. Furthermore, the sequences were detected in only the CD19 + B‐lymphocyte fraction of the patient with LCD as previously reported. However, the sequences were detected in CD19 + B‐lymphocyte and CD2 + T‐lymphocyte fractions of two patients with MCD. These results suggest that HHV‐8 has tropisms for both B lymphocytes and T lymphocytes in Castleman's disease.