Premium
Deletion of a consensus oestrogen response element half‐site in the glucocorticoid receptor of human multiple myeloma
Author(s) -
Karkera Jayaprakash D.,
Taymans Susan E.,
Turner Gordon,
Yoshikawa Takeo,
DeteraWadleigh Sevilla D.,
Wadleigh Robert G.
Publication year - 1997
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1997.4123227.x
Subject(s) - glucocorticoid receptor , allele , biology , glucocorticoid , hormone response element , microbiology and biotechnology , three prime untranslated region , mutant , untranslated region , genetics , single strand conformation polymorphism , gene , messenger rna , mutation , endocrinology , estrogen receptor , breast cancer , cancer
We have carried out molecular scanning of the glucocorticoid receptor (GR) of the glucocorticoid resistant multiple myeloma cell line U266. An amplified fragment from the 3′ untranslated region displayed an aberrant migration by PCR–single‐stranded conformational polymorphism (PCR‐SSCP) analysis. The mutant allele had a deletion of an 8 base pair sequence containing a half‐site of an oestrogen response element. This motif was found conserved in rat GR. This same allele lacked four As in an upstream region with 18 consecutive As in the normal allele. These mutations may affect mRNA stability or alter interactions with regulatory factors.