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mRNA expression of Fas receptor (CD95)‐associated proteins (Fas‐associated phosphatase‐1/FAP‐1, Fas‐associating protein with death domain/FADD, and receptor‐interacting protein/RIP) in human leukaemia/lymphoma cell lines
Author(s) -
Komada Yoshihiro,
Inaba Hiroto,
Zhou YanWen,
Zhang XaoLi,
Tanaka Shigeki,
Azuma Eiichi,
Sakurai Minoru
Publication year - 1997
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1997.3903204.x
Subject(s) - fadd , fas receptor , death domain , fas ligand , microbiology and biotechnology , apoptosis , cell culture , biology , programmed cell death , messenger rna , cancer research , jurkat cells , immunology , t cell , gene , caspase , immune system , biochemistry , genetics
mRNA expression of Fas (CD95)‐associated proteins [Fas‐associating protein with death domain (FADD), receptor‐interacting protein (RIP), and Fas‐associated phosphatase‐1 (FAP‐1)] has been investigated in 26 Fas‐positive human leukaemia/lymphoma cell lines. Reverse transcriptase‐polymerase chain reaction analysis revealed that FADD and RIP mRNA were invariably expressed in both Fas‐sensitive and Fas‐insensitive cell lines. However, FAP‐1 mRNA was detected in only 11 of 26 cell lines. Interestingly 7/14 cell lines in the Fas‐sensitive group were positive for FAP‐1 mRNA expression. 8/12 cell lines in the Fas‐refractory group did not express FAP‐1 mRNA, but half of these cell lines were susceptible to tumour necrosis factor α‐induced growth inhibition. These findings suggest that the presence or absence of FAP‐1 mRNA expression did not always correlate with relative sensitivity of Fas‐mediated growth inhibition. Furthermore, it is assumed that leukaemia/lymphoma cells could possess structural or functional defects of Fas or Fas‐associated proteins resulting in the failure to trigger apoptotic cell death.