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Flow cytometric detection of cytomegalovirus antigen in peripheral blood cells after bone marrow transplantation
Author(s) -
Honda Junichi,
Okubo Yasumitsu,
Imamura Yutaka,
Kusaba Mikako,
Saruwatari Naoko,
Oizumi Kotaro
Publication year - 1997
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1997.3863200.x
Subject(s) - cytomegalovirus , antigen , immunology , bone marrow , flow cytometry , medicine , betaherpesvirinae , pneumonia , peripheral blood , herpesviridae , monocyte , cytomegalovirus infection , human cytomegalovirus , virology , virus , viral disease
Cytomegalovirus (CMV) immediate‐early (IE) antigen within the nucleus of cells was detected by flow cytometry in peripheral blood obtained from five bone marrow transplant recipients. In patients with an unexplained fever, CMV antigens were detected in monocytes or lymphocytes. On the other hand, in patients with CMV pneumonia, CMV antigens were detected in polymorphonuclear leucocytes (PMNLs). We suggest that the detection of CMV antigen in monocytes or lymphocytes may be related to CMV activation or reactivation, and the positive results in PMNLs indicate that the patient has a CMV‐associated disease (CMV pneumonia). Our method may be useful in monitoring CMV activation or reactivation, and analysing the mechanisms of CMV infection.