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Disparate lympho‐erythroid donor to recipient chimaerism in a β°‐thalassaemia bone marrow transplant recipient with red cell indices indicative of apparent full engraftment
Author(s) -
Weinberg R. S.,
Vlachos A.,
Najfeld V.,
Galperin Y.,
Scalise A.,
Fruchtman S.,
Lipton J. M.
Publication year - 1997
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1997.3413154.x
Subject(s) - bone marrow , immunology , hemoglobinopathy , human leukocyte antigen , biology , medicine , antigen , hemolytic anemia
A 4‐year‐old girl with transfusion‐dependent β°‐thalassaemia received an HLA‐identical bone marrow transplant (BMT) from her β°‐thalassaemia trait sister. Prior to BMT, chromosomal analysis revealed the recipient to have 46,XX,9qh+, a polymorphic variant of the heterochromatin region of chromosome 9, which her donor did not have. Within 1 month post‐BMT, 89% of nucleated bone marrow cells were of donor origin. One year later, donor engraftment had decreased to 44% and 34% in nucleated bone marrow cells and blood lymphocytes, respectively. By 2 years, donor lymphocyte engraftment fell to 5%, raising concern of possible graft rejection. To examine erythroid chimaerism, globin synthesis by individual erythroid progenitor cell derived colonies (BFU‐E) was analysed. On days 1000 and 1130 post‐BMT, 79% and 77% of colonies, respectively, synthesized β‐globin and therefore were of donor origin.