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Rat liver biliary epithelial cells support long‐term production of haemopoietic progenitors from human CD34 + cells
Author(s) -
Lamy Isabelle,
Corlu Anne,
Fardel Olivier,
Gandemer Virginie,
Rialland Mickael,
Leberre Claudine,
Le Prise PierreYves,
Fauchet Renee,
Coulombel Laure,
GuguenGuillouzo Christiane
Publication year - 1997
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1997.2813098.x
Subject(s) - progenitor cell , haematopoiesis , biology , cd34 , microbiology and biotechnology , stem cell , stromal cell , cell culture , hepatocyte , immunology , bone marrow , cellular differentiation , liver cytology , cancer research , endocrinology , in vitro , biochemistry , genetics , liver metabolism , gene
In this study we report the supportive activity of rat liver epithelial cells (RLEC) on human haemopoiesis in the absence of exogeneously supplied growth factors. RLEC is a rat cell line derived from primitive biliary cells with epithelial characteristics which induce the long‐term differentiation of hepatocytes through cell–cell contacts. We have established the ability of these cells to sustain long‐term survival and multilineage differentiation of human haemopoietic progenitors from unfractionated bone marrow and growth‐factor mobilized peripheral blood cells, and from human CD34 + and CD34 + CD38 − haemopoietic cells, with a higher efficiency than the murine MS‐5 stromal cell line: the numbers of committed progenitors recovered from RLEC cocultures after 8 weeks were 3‐fold higher than fromMS‐5 cocultures, with an unusually high BFU‐E production. Furthermore, using diffusible insert cultures, we demonstrated that, despite the lack of strong adhesive interaction between haemopoietic cells and RLEC, physical proximity was absolutely required for optimum stimulation of LTC‐IC by RLEC. Taken together, these results show that biliary epithelial cells support human haemopoiesis and cause speculation that common mechanisms might be used by RLEC to regulate both the hepatocyte and the haemopoietic progenitors differentiation.