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Quantitative PCR of the immunoglobulin heavy chain gene using genomic DNA
Author(s) -
ELSWORTH A. M.,
EVANS P. A. S.,
MORGAN G. J.,
KINSEY S. E.,
SHIACH C. R.
Publication year - 1996
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1996.d01-1950.x
Subject(s) - immunoglobulin heavy chain , polymerase chain reaction , genomic dna , dna , gene , antibody , genetics , heavy chain , immunoglobulin gene , biology , computational biology
Techniques currently available enable the detection of clonal rearrangements of the immunoglobulin heavy chain gene using fluorescent PCR technology. It is possible to use this technique to analyse minimal residual disease throughout patient treatment; however, without the development of a quantitative assay, only the presence or absence of a clonal population can be determined. We describe here the development of a quantitative competitive PCR technique using genomic DNA which enables the rate of clearance of disease to be measured. In future, the ability to detect and also quantitate minimal residual disease may enhance the application of molecular investigations in the clinical management of patients.