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A (15;17) translocation not associated with acute promyelocytic leukaemia
Author(s) -
Di Bona Eros,
Montaldi Anna,
Guercini Nicola,
Rossi Vincenzo,
Luciano Anna,
Biondi Andrea,
Rodeghiero Francesco
Publication year - 1996
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1996.d01-1949.x
Subject(s) - chromosomal translocation , fluorescence in situ hybridization , breakpoint , retinoic acid , acute promyelocytic leukemia , fusion gene , biology , cancer research , fish <actinopterygii> , cytogenetics , microbiology and biotechnology , gene , genetics , chromosome , fishery
We report a woman with acute myeloid leukaemia (AML) type M2 according to FAB classification, showing a t(15;17) apparently identical to that of acute promyelocytic leukaemia (APL) on conventional cytogenetic analysis. Fluorescence in situ hybridization (FISH) using cosmidic probes specific for RARα and PML regions did not show a fusion signal as in APL. The breakpoints were assigned to 15q24.3 and 17q21.1. Detailed molecular analyses did not reveal any involvement of RARα and PML genes. The patient was resistant to several front‐line AML treatments and to all‐ trans retinoic acid (ATRA). These findings reinforce FISH and RT‐PCR as useful tools for the characterization of a t(15;17) as the translocation specifically associated with APL.