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Recombinant human c‐Mpl ligand is not a direct stimulator of proplatelet formation in mature human megakaryocytes
Author(s) -
ITO TOSHIHARU,
ISHIDA YOJI,
KASHIWAGI RIEKO,
KURIYA SHINICHIRO
Publication year - 1996
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1996.d01-1813.x
Subject(s) - ligand (biochemistry) , thrombopoietin , recombinant dna , megakaryocyte , platelet , thrombopoiesis , in vitro , erythropoietin , chemistry , microbiology and biotechnology , biology , receptor , immunology , biochemistry , endocrinology , haematopoiesis , stem cell , gene
To evaluate the effect of the c‐Mpl ligand on platelet production by megakaryocytes, we investigated proplatelet formation in isolated human megakaryocytes cultured in serum‐free medium, with or without the c‐Mpl ligand, interleukin‐6 and erythropoietin. When interleukin‐6 was added to the culture medium, the percentage of megakaryocytes displaying proplatelets was approximately 1.5‐fold the control value; whereas, in the presence of the c‐Mpl ligand, the percentage of megakaryocytes displaying proplatelets decreased in a dose‐dependent manner and did not increase compared to control values at any dose tested. However, the viability of megakaryocytes after a 4 d culture in the presence of the c‐Mpl ligand was significantly higher than that of the cells cultured without it. The c‐Mpl ligand did not stimulate the proplatelet formation in megakaryocytes in vitro