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Clonal expansion of γδ T cells expressing two distinct T‐cell receptors
Author(s) -
Hinz Thomas,
Marx Sibylle,
Nerl Christoph,
Kabelitz Dieter
Publication year - 1996
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1996.d01-1779.x
Subject(s) - microbiology and biotechnology , t cell receptor , biology , delta , monoclonal antibody , population , gamma delta t cell , t cell , complementary dna , receptor , t lymphocyte , antibody , immunology , antigen , genetics , gene , medicine , physics , immune system , environmental health , astronomy
92% of peripheral blood mononuclear cells from a patient with chronic lymphocytosis (WBC 22×10 9 /l) were recognized by pan‐γδ monoclonal antibody (mAb) TCRδ1. Further analysis with anti‐Vγ/Vδ mAb indicated that the expanded γδ T‐cell population was stained by mAb specific for Vγ2/3/4, Vγ5 and Vδ1 but not mAb specific for Vγ8, Vγ9, Vδ2 or Vδ3. Cloning and sequencing of reverse transcription PCR products revealed the presence of Vγ4, Vγ5 and Vδ1 in‐frame transcripts. All the cDNA clones obtained from each of these variable elements had identical junction regions, thus clearly demonstrating clonality. As far as we know, this is the first description of a pathological clonal expansion of γδ T cells with two distinct surface‐expressed T‐cell receptor molecules.