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p53 tumour suppressor gene and RAS oncogenes: molecular analysis in the chronic and leukaemic phases of essential thrombocythaemia
Author(s) -
NERI ANTONINO,
FRACCHIOLLA NICOLA STEFANO,
RADAELLI FRANCA,
BOLETINI ALKETA,
RIBERA SONIA,
MIGLIORINI CHIARA,
TRECCA DINO,
MAIOLO ANNA TERESA
Publication year - 1996
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1996.d01-1690.x
Subject(s) - gene , cancer research , pathogenesis , mutation , chemotherapy , tumor suppressor gene , biology , suppressor , medicine , carcinogenesis , immunology , genetics
A panel of 51 cases of essential thrombocythaemia (ET), in chronic or leukaemic phase, was investigated for p53 gene and RAS oncogenes mutations by PCR‐SSCP‐direct sequencing. No RAS oncogenes mutations were detected, but p53 mutations were identified in three cases: 1/27 cases (≈4%) in chronic phase not undergoing chemotherapy, 1/19 cases (≈5%) in chronic phase undergoing chemotherapy, and 1/5 cases (20%) which had progressed to leukaemia. Our results suggest that: (1) p53 gene mutations occur sporadically in the chronic phase of ET, independent of chemotherapy, and may contribute to the progression to the leukaemic phase in a limited number of ET patients; (2) the RAS genes family does not seem to be involved in the pathogenesis of ET, unlike other bcr/abl negative chronic myeloproliferative diseases (CMPDs).