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Molecular diagnosis of t(11;14) in mantle cell lymphoma using two‐colour interphase fluorescence in situ hybridization
Author(s) -
MONTEIL MICHÈLE,
CALLANAN MARY,
DASCALESCU CRISTINA,
SOTTO JEANJACQUES,
LEROUX DOMINIQUE
Publication year - 1996
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1996.d01-1675.x
Subject(s) - mantle cell lymphoma , fluorescence in situ hybridization , interphase , chromosomal translocation , breakpoint , microbiology and biotechnology , cytogenetics , in situ hybridization , biology , lymphoma , fish <actinopterygii> , pathology , chromosome , genetics , medicine , gene , messenger rna , immunology , fishery
t(11;14) is observed in up to 70% of mantle cell lymphoma (MCL) cases and is therefore an important diagnostic element. In routine practice, detection of t(11;14) by conventional cytogenetic techniques is hindered by the low yield and quality of tumour metaphases. Molecular techniques (Southern blot, PCR) are unable to detect a large number of 11q13 breakpoints due to scattering over distances up to 1 Mb. Using 23 MCL patients with karyotypically determined t(11;14) and eight negative controls, we have devised a two‐colour interphase FISH assay for detection of the 14q+ chromosome. We chose an 11q13 probe telomeric to the major 11q13 translocation cluster sites and an IGH probe centromeric of the 14q32 breakpoints. This method detected the translocation in all 23 t(11;14) positive patients, with an overall average of 60% nuclei showing colocalized signals. Widespread application of this technique will constitute an important diagnostic aid in clinical management of MCL patients. Since FISH is a convenient method for retrospective analysis of large numbers of patient specimens, this method should contribute to an accurate estimation of t(11;14) frequency in MCL and other chronic B‐cell malignancies and consequently to their better nosological characterization.