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Detection of microsatellite markers in leukaemia using DNA from archival bone marrow smears
Author(s) -
Pabst Thomas,
Schwaller Jürg,
Tobler Andreas,
Fey Martin F.
Publication year - 1996
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1996.7622385.x
Subject(s) - microsatellite , loss of heterozygosity , bone marrow , buccal swab , biology , pathology , dna , dna extraction , str analysis , polymerase chain reaction , microbiology and biotechnology , genetics , medicine , gene , allele
We describe a simple PCR method to detect highly polymorphic repetitive DNA markers (microsatellites) in leukaemia using DNA from fresh and archival bone marrow smears. Neither the period of storage nor staining of the slides affected microsatellite PCR. Comparison of slide DNA with constitutive DNA from buccal epithelium revealed loss of heterozygosity (LOH) with at least one marker in four of 11 leukaemias. Marrow smears offer an attractive archival source of DNA for the detection of microsatellite markers. Applications include detection of LOH or tracing the origin of cell populations, e.g. in bone marrow transplant recipients.