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Multiplex PCR for rapid detection of T‐cell receptor‐gamma chain gene rearrangements in patients with lymphoproliferative diseases
Author(s) -
Födinger Manuela,
Buchmayer Heidi,
Schwarzinger Ilse,
Simonitsch Ingrid,
Winkler Karin,
Jäger Ulrich,
Knobler Robert,
Mannhalter Christine
Publication year - 1996
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1996.6372268.x
Subject(s) - t cell receptor , multiplex polymerase chain reaction , lymphocytosis , polymerase chain reaction , gene rearrangement , primer (cosmetics) , lymphoproliferative disorders , multiplex , biology , microbiology and biotechnology , gene , immunology , t cell , genetics , chemistry , lymphoma , immune system , organic chemistry
We describe a multiplex polymerase chain reaction (PCR) method suitable for the detection of all T‐cell receptor (TCR) γ‐chain gene rearrangements in patients with lymphoproliferative diseases. 40 patients with various lymphoproliferative disorders and 40 healthy individuals were tested. Clonal TCRγ rearrangements were identified in all patients with malignant disorders, and in one of 10 cases with established reactive lymphocytosis but not in normal controls. In all individuals testing positive, the patient’s specific V and J segment involved in the rearrangement could be determined by simply splitting the multiplex primer mix. Our data show that the multiplex PCR technique enables rapid, simple and sensitive screening for clonal TCRγ chain gene rearrangements.