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Heparin induces apoptosis in human peripheral blood neutrophils
Author(s) -
Manaster J.,
Chezar J.,
ShurtzSwirski R.,
Shapiro G.,
Tendler Y.,
Kristal B.,
Shasha S. M.,
Sela S.
Publication year - 1996
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1996.6202063.x
Subject(s) - tunel assay , terminal deoxynucleotidyl transferase , apoptosis , flow cytometry , heparin , trypan blue , in vitro , programmed cell death , microbiology and biotechnology , immunology , biology , chemistry , biochemistry
Programmed cell death, by apoptosis, has been shown to play an important role in the regulation of haemopoiesis. Using trypan blue exclusion for distinguishing intact membranes, flow cytometry for detection of sub G1 peak and in situ terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (TUNEL), this study shows that heparin induces apoptosis in vitro in human peripheral blood neutrophils. The known anti‐proliferative effect of heparin in several in vitro cell systems has therefore to be interpreted in the light of apoptosis. In addition, apoptosis may help explain the anti‐inflammatory effects resulting from the interaction between vessel wall heparan sulphate and chemoattracted peripheral blood neutrophils.