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Detection of donor cell derived acute myelogenous leukaemia in a patient transplanted for chronic myelogenous leukaemia using fluorescence in situ hybridization
Author(s) -
Lowsky Robert,
Fyles Gillian,
Minden Mark,
Lipton Jeffrey,
Meharchand Jacinta,
Tejpar Ismet,
Zipursky Alvin,
Messner Hans
Publication year - 1996
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1996.454991.x
Subject(s) - fluorescence in situ hybridization , chronic myelogenous leukemia , bone marrow , transplantation , metaphase , cytogenetics , pathology , medicine , leukemia , biology , chromosome , cancer research , immunology , genetics , gene
The recurrence of leukaemia following allogeneic bone marrow transplantation appears to develop rarely in donor cells. However, the standard method for assigning the origin of recurrence, metaphase analysis, can be unreliable. We have applied the technique of fluorescence in situ hybridization (FISH) directly on archival Wright stained bone marrow slides obtained from a patient who developed acute myelogenous leukaemia (AML) following allogeneic bone marrow transplantation (BMT) for chronic myelogenous leukaemia (CML). Using a chromosome‐specific DNA probe we linked a chromosomal aberration, previously detected by conventional metaphase analysis, directly to morphologically identifiable blast cells. In this way we were able to assess cell‐lineage involvement of the secondary leukaemia and assign a donor origin.