Premium
Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia
Author(s) -
Marukawa Osamu,
Akao Yukihiro,
Inazawa Johji,
Ariyama Takeshi,
Abe Tatsuo,
Naoe Tomoki,
Tanimoto Mitsune,
Saito Hidehiko,
Otsuki Yoshinori,
Tsujimoto Yoshihide
Publication year - 1996
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1996.380916.x
Subject(s) - locus (genetics) , breakpoint , microbiology and biotechnology , chromosomal translocation , biology , southern blot , genetics , complementary dna , exon , gene , breakpoint cluster region
Southern blot analysis with a cDNA probe of MLL indicated that the breakpoint is in a Bam HI 8.3 kb fragment which carries the exon 5–11 of MLL gene in DNA from an adult acute myelomonocytic leukaemia with a t(11;22)(q23;q11) translocation. The structural analysis of the rearranged MLL locus demonstrated that the breakpoint is localized between exon 8 and 9 of MLL locus. The normal counterpart fused to the MLL locus was proved to be derived from chromosome 22q11( AF‐22 ) by somatic cell hybrids analysis and FISH. By FISH, AF‐22 locus was localized to the region more centromeric to the BCR gene.