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Interleukin‐10 mRNA expression in B‐cell chronic lymphocytic leukaemia inversely correlates with progression of disease
Author(s) -
Sjöberg Jan,
Aguilar Santelises Miguel,
Sjögren AnnMarie,
Pisa Eva K.,
Ljungdahl ÅKe,
Björkholm Magnus,
Jondal Mikael,
Mellstedt HÅkan,
Pisa Pavel
Publication year - 1996
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1996.00358.x
Subject(s) - chronic lymphocytic leukemia , messenger rna , b cell , biology , interleukin 9 , immunology , progressive disease , microbiology and biotechnology , immune system , interleukin , cancer research , medicine , disease , leukemia , pathology , cytokine , antibody , gene , genetics
Interleukin‐10 (IL‐10) has been shown in vitro to inhibit survival and spontaneous DNA synthesis in B‐cell chronic lymphocytic leukaemia (B‐CLL) cells by induction of programmed cell death. We have analysed the presence of mRNA transcripts for IL‐10 in purified B‐CLL cells from 35 patients by RT‐PCR. Transcripts for IL‐10 were detected in 11/20 patients with non‐progressive disease. In cell preparations from patients with progressive B‐CLL IL‐10 mRNA were detected in only 2/15 samples ( P ≤ 0.01). The Epstein‐Barr virus status of the cells did not account for the difference in IL‐10 mRNA expression observed between the two groups of patients. Thus, IL‐10 mRNA expression in leukaemic cells from patients with B‐CLL was strongly associated with non‐progressive disease. This finding may support other observations suggesting that IL‐10 might be a candidate for immune therapy of progressive B‐CLL.