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Direct stimulatory effect of low‐intensity 670 nm laser irradiation on human endothelial cell proliferation
Author(s) -
Schindl A.,
Merwald H.,
Schindl L.,
Kaun C.,
Wojta J.
Publication year - 2003
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1046/j.1365-2133.2003.05070.x
Subject(s) - cell growth , umbilical vein , irradiation , endothelial stem cell , human umbilical vein endothelial cell , angiogenesis , wound healing , laser , cell , intensity (physics) , in vivo , hemocytometer , chemistry , medicine , biology , pathology , immunology , cancer research , optics , in vitro , biochemistry , physics , microbiology and biotechnology , nuclear physics
Summary Background Endothelial cell (EC) proliferation plays a key role in the process of tissue repair. Low‐intensity laser irradiation has been demonstrated to accelerate wound healing and to improve microvascularization. Objectives The present study evaluated a possible stimulatory influence of low‐intensity laser irradiation on human umbilical vein endothelial cell (HUVEC) proliferation in a systematic manner. Methods Subconfluent cultures of HUVEC were irradiated every other day with a 670‐nm diode laser (intensity: 10–65 mW cm −2 , dose: 2–8 J cm −2 ) during a period of 6 days. Cell proliferation was evaluated quantitatively by counting in a haemocytometer. Results Our data demonstrate a dose‐dependent and intensity‐dependent stimulatory effect of laser irradiation on HUVEC cell proliferation. Doses of between 2 and 8 J cm −2 induced statistically significant cell proliferation. Testing different intensities at a constant dose of 8 J cm −2 , 20 and 65 mW cm −2 induced most pronounced cell proliferation. Conclusions Low‐intensity laser irradiation influences EC proliferation and might thereby contribute to the increase in angiogenesis and the acceleration of wound healing in vivo .