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Spatial and temporal expression of basic fibroblast growth factor protein during wound healing of rat skin
Author(s) -
Kibe Y.,
Takenaka H.,
Kishimoto S.
Publication year - 2000
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1046/j.1365-2133.2000.03824.x
Subject(s) - granulation tissue , wound healing , immunostaining , basic fibroblast growth factor , epidermis (zoology) , pathology , angiogenesis , neovascularization , biology , fibroblast , fibroblast growth factor , immunohistochemistry , microbiology and biotechnology , growth factor , medicine , immunology , anatomy , cancer research , cell culture , biochemistry , receptor , genetics
Background Basic fibroblast growth factor (bFGF) stimulates the mitogenesis of various cells and plays a key part in wound healing. Objectives To determine the spatial and temporal expression of bFGF protein during wound healing after burning of rat skin. Methods Immunohistochemical methods were used. Results The immunostaining for bFGF in the normal epidermis was faint and sporadic in the basal cell layer. However, significant staining for bFGF was found in four locations: regenerated epidermis, a band‐like zone near the regenerated epidermis, renewed capillaries, and cells infiltrating into the granulation tissue at the inflammatory to proliferative stages after the burn. The intensity of immunostaining of regenerated epidermis, the band‐like zone and renewed capillaries was maximal during the proliferative stage and decreased to normal levels or disappeared simultaneously with wound closure. Immunopositive macrophage‐like cell numbers in the granulation tissue increased during the proliferative stage and promptly decreased after wound closure, but such cells were only poorly visible in the scar tissue until 42 days postburn. Conclusions bFGF may affect the proliferation, differentiation and migration of regenerated keratinocytes and the recruitment of inflammatory cells, as well as neovascularization in granulation tissue during wound healing. Macrophages may play a pivotal role in cutaneous wound repair by producing bFGF not only during the inflammatory or proliferative stages but also during the remodelling stage.