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Elevated levels of eotaxin and interleukin‐5 in blister fluid of bullous pemphigoid: correlation with tissue eosinophilia
Author(s) -
Wakugawa M.,
Nakamura K.,
Hino H.,
Toyama K.,
Hattori N.,
Okochi H.,
Yamada H.,
Hirai K.,
Tamaki K.,
Furue M.
Publication year - 2000
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1046/j.1365-2133.2000.03599.x
Subject(s) - eotaxin , eosinophil , eosinophilia , bullous pemphigoid , chemokine , immunology , immunohistochemistry , interleukin 5 , medicine , infiltration (hvac) , interleukin , pathology , cytokine , inflammation , antibody , asthma , physics , thermodynamics
Background Bullous pemphigoid (BP) often provokes blood and tissue eosinophilia, which suggests that some chemoattractants modulate the eosinophil infiltration in BP. Eotaxin, a CC chemokine, strongly attracts eosinophils, and interleukin (IL)‐5 induces eosinophil differentiation, proliferation and colony formation in vitro . Objectives To examine the correlation between levels of eotaxin and IL‐5 and the number of lesional eosinophils, and the expression of eotaxin in BP lesions. Patients/methods In this study we measured eotaxin and IL‐5 levels in blister fluid of BP by enzyme‐linked immunosorbent assay. We also examined the expression of eotaxin in BP lesions by immunohistochemistry. Results Both eotaxin and IL‐5 were detected at high levels in BP blister fluid. Blister fluid eotaxin, but not IL‐5 levels, correlated significantly with the number of dermal infiltrating eosinophils. By immunohistochemistry, eotaxin was strongly expressed in epidermal keratinocytes around BP blisters. Conclusions These findings suggest that eotaxin and IL‐5 are strongly associated with the tissue eosinophilia of BP. Therapies which aim to inhibit production of eotaxin and IL‐5 may improve the inflammation and blister formation in BP.