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Psoralen‐ultraviolet A‐induced erythema: sensitivity correlates with the concentrations of psoralen in suction blister fluid
Author(s) -
Yeo UC.,
Shin JH.,
Yang JM.,
Park KB.,
Kim MM.,
Bok HS.,
Lee ES.
Publication year - 2000
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1046/j.1365-2133.2000.03419.x
Subject(s) - psoralen , erythema , puva therapy , suction blister , chemistry , ultraviolet , chromatography , dermatology , psoriasis , medicine , surgery , materials science , biochemistry , dna , optoelectronics
Since the advent of psoralen‐ultraviolet A (PUVA) therapy, the value of plasma 8‐methoxypsoralen (8‐MOP) concentrations to predict PUVA‐induced erythema has been widely investigated. Plasma 8‐MOP concentrations have not been proportional to, and cannot alone predict, the degree of PUVA‐induced erythema. We assumed that PUVA‐induced erythema was related more closely to psoralen concentrations in the skin tissue rather than those within blood vessels. This study was designed to investigate the correlations between the 8‐MOP concentrations in suction blister fluid (SBF) and in plasma, with the degree of PUVA‐induced erythema. 8‐MOP concentrations in plasma and SBF were measured in 15 vitiligo patients and 11 volunteers. Blood and SBF samples were collected 2 h after taking 8‐MOP, and 8‐MOP concentrations in plasma and SBF were quantified using reverse‐phase high‐performance liquid chromatography. Eleven volunteers were phototested using a series of doses of ultraviolet A at the time of sampling. The erythema responses were estimated visually to determine the minimal phototoxic dose (MPD). SBF 8‐MOP concentrations showed a weak positive correlation with plasma 8‐MOP concentrations, which means that we could not predict the exact SBF 8‐MOP concentrations using the plasma 8‐MOP concentrations. The MPD showed a better correlation with the log of the SBF 8‐MOP concentration than with that of the plasma 8‐MOP concentration. These results show that plasma 8‐MOP concentration cannot represent the exact SBF 8‐MOP concentration, and that SBF 8‐MOP concentrations, which are representative of the skin tissue 8‐MOP level, are more closely related to the erythemal sensitivity during PUVA therapy.