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Fibroblast expression of collagen integrin receptors α1β1 and α2β1 is not changed in systemic scleroderma
Author(s) -
Katja Herzhoff,
Stephan Sollberg,
Christina Huerkamp,
Thomas Krieg,
Beate Eckes
Publication year - 1999
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1046/j.1365-2133.1999.02968.x
Subject(s) - scleroderma (fungus) , integrin , fibroblast , receptor , systemic scleroderma , systemic disease , dermal fibroblast , medicine , microbiology and biotechnology , immunology , pathology , biology , immunopathology , biochemistry , in vitro , inoculation , dermatomyositis
The skin of patients with systemic scleroderma (SSc) is characterized by excessive extracellular matrix deposition in the dermis. As collagens represent the major structural component, we used fluorescence‐activated cell sorter analysis to study the levels of collagen receptors expressed at the surface of fibroblasts derived from involved skin areas. In contrast to previous reports, no differences in the expression of α1, α2 or β1 integrin subunits, which constitute the major collagen receptors on fibroblasts, were detected on SSc fibroblasts as compared with normal control fibroblasts. Variation of cell culture conditions, e.g. passage number (from 2 to 10), seeding density, cell cycle or serum concentration, did not change this result. These observations indicate that any abnormal response of SSc fibroblasts to their matrix environment is not controlled at the level of receptor expression.