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In vitro modulation of keratinocyte wound healing integrins by zinc, copper and manganese
Author(s) -
Isabelle Tenaud,
Isabelle Sainte-Marie,
O Jumbou,
P Litoux,
Brigitte Dréno
Publication year - 1999
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1046/j.1365-2133.1999.02603.x
Subject(s) - integrin , zinc , keratinocyte , chemistry , wound healing , in vivo , copper , in vitro , microbiology and biotechnology , receptor , biochemistry , immunology , biology , organic chemistry
Although the trace elements zinc, copper and manganese are used in vivo for their healing properties, their mechanism of action is still only partially known. Some integrins expressed by basal layer keratinocytes play an essential part in healing, notably α2β1, α3β1, α6β4 and αVβ5, whose expression and distribution in epidermis are modified during the re‐epithelialization phase. This study demonstrates how the expression of these integrins are modulated in vitro by trace elements. Integrin expression was studied in proliferating keratinocytes in monolayer cultures and in reconstituted skin that included a differentiation state. After 48 h incubation with zinc gluconate (0.9, 1.8 and 3.6 μg/mL), copper gluconate (1, 2 and 4 μg/mL), manganese gluconate (0.5, 1 and 2 μg/mL) and control medium, integrin expression was evaluated by FACScan and immunohistochemistry. Induction of α2, α3, αV and α6 was produced by zinc gluconate 1.8 μg/mL in monolayers, of α2, α6 and β1 by copper gluconate 2 and 4 μg/mL and of all the integrins studied except α3 by manganese gluconate 1 μg/mL. Thus, α6 expression was induced by all three trace elements. The inductive effect of zinc was particularly notable on integrins affecting cellular mobility in the proliferation phase of wound healing (α3, α6, αV) and that of copper on integrins expressed by suprabasally differentiated keratinocytes during the final healing phase (α2, β1 and α6), while manganese had a mixed effect.