Premium
Proliferation and differentiation of organoid hair follicle cells co‐cultured with fat cells in collagen gel matrix culture
Author(s) -
Noriyuki Misago,
Shuji Toda,
Hajime Sugihara,
Hiromu Kohda,
Yutaka Narisawa
Publication year - 1998
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1046/j.1365-2133.1998.02312.x
Subject(s) - hair follicle , organoid , microbiology and biotechnology , cellular differentiation , cell culture , cell growth , matrix (chemical analysis) , chemistry , cell type , biology , endocrinology , medicine , cell , biochemistry , genetics , chromatography , gene
Using rat skin, we studied the influence of fat cells on the proliferation and differentiation of organoid hair follicle cells in a three‐dimensional collagen gel matrix culture system. We cultured organoid hair follicles embedded in collagen gel under each of the following three conditions: cell‐free collagen gel for control experiments (condition 1); co‐culture with fat cells in close apposition (condition 2); and co‐culture with fat cells in spatial separation (condition 3). Outgrowths of epithelial cells from the organoid hair follicles associated with perifollicular proliferation of fibroblasts were observed under conditions 1 and 3. Under condition 2, proliferation of both organoid hair follicle cells and fibroblasts was inhibited, but differentiation of the hair follicle cells appeared to be accelerated. Fat cells are considered to have an inhibitory effect on the proliferation of perifollicular fibroblasts, which might have resulted in the inhibition of hair follicle cell proliferation and also in the better maintenance of normal follicular structure and integrity, allowing for hair‐type differentiation to proceed. A direct accelerating effect of fat cells on hair follicle differentiation may also have been responsible. In a physiological state (co‐culture with keratinocytes on the collagen gel), similar results were observed under conditions 1 and 2. The different findings under conditions 2 and 3 may be due to either of two possibilities: either the concentration gradient of the soluble factors released from fat cells, acting on either the hair follicle cells or the perifollicular fibroblasts as an inhibitor of proliferation, caused the difference in the results, or direct contact between the organoid hair follicle cells and fat cells may have influenced the accelerating effect of fat cells on the differentiation of hair follicle cells.