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Regulation of matrix metalloproteinase (MMP) expression in cutis laxa fibroblasts: upregulation of MMP‐1, MMP‐3 and MMP‐9 genes but not of the MMP‐2 gene
Author(s) -
Atsushi Hatamochi,
Kensuke Kuroda,
Hiroshi Shinkai,
H Kohma,
Yuichi Oishi,
Shintaro Inoue
Publication year - 1998
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1046/j.1365-2133.1998.02210.x
Subject(s) - matrix metalloproteinase , gelatinase a , gene expression , downregulation and upregulation , western blot , microbiology and biotechnology , northern blot , fibroblast , messenger rna , matrix metalloproteinase 3 , biology , gene , metalloproteinase , blot , cutis laxa , pathology , chemistry , medicine , cell culture , biochemistry , genetics
A major histopathological abnormality in cutis laxa (CL) is a paucity of elastic structures. The aim of this study was to investigate the gene expression levels of the major matrix degrading factors matrix metalloproteinase (MMP) 1, MMP‐2, MMP‐3 and MMP‐9 in CL. The gene expression levels of MMP‐1, MMP‐2, MMP‐3 and MMP‐9 in cultured CL fibroblasts were measured by northern blot, immunoblot and gelatin zymographic analysis. Markedly increased mRNA levels of MMP‐1 (8.4‐fold), MMP‐3 (7.2‐fold) and MMP‐9 (more than 10‐fold) were found in CL fibroblasts, whereas MMP‐2 mRNA levels in these fibroblasts were unaltered. Increased protein production levels of MMP‐1 (4.6‐fold) and MMP‐3 (5.1‐fold) in CL fibroblasts were shown by immunoblot analysis. On gelatin zymographic analysis, the gelatinolytic activities of MMP‐9 but not of MMP‐2 were increased (2.2‐fold). These results suggest that increased gene expression levels of MMP‐1, MMP‐3 and MMP‐9 in CL fibroblasts may contribute to the histopathological abnormality in CL.