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Nodular localized cutaneous amyloidosis: further demonstration of monoclonality of infiltrating plasma cells in four additional Japanese patients
Author(s) -
Yoshitaka Hagari,
Motoyuki Mihara,
Izumi Konohana,
Hiroaki Ueki,
Osamu Yamamoto,
Hiroko Koizumi
Publication year - 1998
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1046/j.1365-2133.1998.02179.x
Subject(s) - nested polymerase chain reaction , immunoglobulin light chain , pathology , al amyloidosis , amyloidosis , polymerase chain reaction , antibody , monoclonal , plasma cell , amyloid fibril , amyloid (mycology) , monoclonal antibody , chemistry , medicine , biology , immunology , disease , genetics , gene , amyloid β , bone marrow
Nodular localized cutaneous amyloidosis (NLCA) is a disorder characterized by deposition of amyloid derived from immunoglobulin light chains. We used semi‐nested polymerase chain reaction (PCR) to analyse archival paraffin‐embedded sections from a previous patient and from four additional, previously reported patients with NLCA to determine whether involvement of monoclonal plasma cells is a universal feature of this condition. The semi‐nested PCR analysis revealed one or two amplified bands, around 100–120 bp, for all five cases of NLCA, although the yields varied from case to case. These results suggest that clonal expansion of plasma cells in NLCA may occur locally.