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Opposite effects of tumour necrosis factor‐α on type I and III collagen gene expression by human dermal fibroblasts in monolayer and three‐dimensional cultures
Author(s) -
Madoka Sato,
Osamu Ishikawa,
Abe M,
Yoshiki Miyachi
Publication year - 1998
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1046/j.1365-2133.1998.02036.x
Subject(s) - dermal fibroblast , fibroblast , extracellular matrix , ascorbic acid , dermis , tumor necrosis factor alpha , gene expression , type i collagen , microbiology and biotechnology , matrix metalloproteinase , biology , extracellular , cell culture , necrosis , chemistry , biochemistry , immunology , endocrinology , gene , anatomy , genetics , food science
We have recently established a novel fibroblast culture system supplemented with l ‐ascorbic acid 2‐phosphate. The addition of l ‐ascorbic acid 2‐phosphate enables human dermal fibroblasts to organize a three‐dimensional dermis‐like structure by accumulating collagens and extracellular matrices. The purpose of this study was to examine the effects of tumour necrosis factor‐α (TNF‐α) on collagen gene expression by human dermal fibroblasts in this culture system in comparison with monolayer culture. TNF‐α suppressed the expression of proα1(I) and proα1(III) collagen mRNA in monolayer culture. In contrast, their expression was elevated in the three‐dimensional culture system. TNF‐α increased the mRNA expression of matrix metalloproteinase‐1 and tissue inhibitor of metalloproteinase‐1 both in monolayer and three‐dimensional culture. These data suggest that responses of human dermal fibroblasts to TNF‐α are distinct under the different culture conditions. Extracellular matrices may modulate the responsiveness of fibroblasts to TNF‐α.