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Altered expression of the hemidesmosome‐anchoring filament complex proteins in basal cell carcinoma: possible role in the origin of peritumoral lacunae
Author(s) -
BAHADORAN Ph.,
PERRIN Ch.,
ABERDAM D.,
SPADAFORAPISANI A.,
MENEGUZZI G.,
ORTONNE JP.
Publication year - 1997
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1046/j.1365-2133.1997.d01-1139.x
Subject(s) - plectin , hemidesmosome , laminin , integrin , basement membrane , pathology , immunostaining , biology , antigen , microbiology and biotechnology , chemistry , intermediate filament , immunohistochemistry , cell , immunology , cytoskeleton , extracellular matrix , medicine , biochemistry
Summary Basal cell carcinoma (BCC) is a frequent skin cancer with low metastalic potential. Expression of the anchoring filament proteins, native laminin‐5 and its individual α 3 , β 3 and γ 2 chains, uncein, and linear IgA antigen was examined by immunostaining in 17 BCC with different histological subtypes. Immunoreactivity of the hemidesmosomal proteins, integrin α 6 β 4 . 230‐kDa bullous pemphigoid antigen (BP‐230 Ag) and plectin/HD‐1, and that of dermal‐epidermal junction (DEJ) components, integrin α 2 β 1 , laminin‐1, collagen IV, and collagen VII was also analysed. Around tumour nests, the labelling of laminin‐5 was absent or markedly reduced in 12 BCC (comprising eight solid BCC, three adenoid BCC and one keratotic BCC) and strong in five BCC (comprising three adenoid BCC, one keratotic BCC and one adenoid and keratotic BCC). Intriguingly, in tumour cells of 12 BCC including laminin‐5 negative tumours, a cytoplasmic reactivity of the laminin 72 chain was detected, but not that of the α 3 and β 3 chains. In the basement membrane of the epidermis overlying tumour nests, the labelling of laminin‐5 was always strong. Uncein, linear IgA disease antigen, and integrin α 6 β 4 were absent in solid BCC and weakly expressed in adenoid or keratotic BCC. For plectin/HD‐1 and BP‐230 Ag, a cytoplasmic reactivity was detected in the majority of the tumour cells. The labelling of integrin α 2 β 1 , laminin‐1, collagen IV and collagen VII indicated no alteration in the synthesis of these proteins. In peritumoral lacunae, immunoreactivity of hemidesmosome and anchoring filament proteins was absent, except for plectin/HD‐1 on the tumour side and sometimes for laminin‐5 on the stromal side, while laminin‐1, collagen IV and collagen VII were detected on the stromal side. These findings suggest that the components of the hemidesmosome‐anchoring filament complex are not synthetized or assembled properly in BCC, and that the alteration of these adhesion structures may be the cause of peritumoral lacunae.